The early renal injury diagnostic value of new markers joint detection for type 2 diabetic kidney injury / 重庆医学

ABSTRACT

Objective To explore the diagnosis value of urinary molecular markers of Kidney injury molecule 1(Kim-1),heat shock protein 72(Hsp72),plasminogen activator inhibitor 1(PAI-1),neutrophil gelatinase associated lipocalin(NGAL)joint detec-tion in the diagnosis of early type 2 diabetes renal damage.Methods Totally 187 cases of type 2 diabetes mellitus patients were sep-arate into two groups based on combination of four urine proteins[Microalbumin(MA),urinary transferrin(TRU),urine immuno-globulin(IGU),urine alpha 1 microglobulin (A1M)].Group A had 48 cases,and the urine protein of patients were negative.Group B had 139 cases,and the urine protein of patients were positive.The normal control group(NC group)selected 50 cases of urinary protein negative healthy people.MA,TRU,IGU,A1M were detected by rate scattering turbidimetric method,and Kim-1,Hsp72, PAI-1and NGAL were detected by enzyme-linked immunosorbent.Results Compared with NC group,the levels of urine Kim-1, Hsp72 and PAI-1 had significant increase in group A(P 0.5).The levels of Kim-1,Hsp72,PAI-1 and NGAL significantly increased in group B(P <0.01),and the increase value was more significantly in group B(P <0.01).The positive rates l of Kim-1,Hsp72,PAI-1 and NGAL joint detection reached 66.70% in group A,which was significantly higher than single detection(P <0.01 ).Conclusion The levels of Hsp72,PAI-1,Kim-1 NGAL protein have been a significant exception before urinary protein in type 2 diabetes mellitus patients,and the abnormal is obvious with the increase of renal damage degree.Kim-1,Hsp72,PAI-1,NGAL can be used as early biomarkers of kidney injury in type 2 diabe-tes,the joint detection will greatly increase the positive detection rate.