Effects of Intra-articular Injection of Sinomenine on Morphology, MMP-13 Level and Cartilage Oligomeric Matrix Protein of Rabbit Knee Osteoarthritis Model / 中国中医药信息杂志

ABSTRACT

Objective To observe the effects of intra-articular injection of sinomenine on morphology of synovium and cartilage as well as contents of MMP-13 and cartilage oligomeric matrix protein(COMP) in serum and synovial fluid of rabbit knee osteoarthritis model.Methods A total of 38 New Zealand white rabbits were randomly divided into 4 groups control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by intra-articular injection of papain enzyme, and were treated with saline, sodium hyaluronate and sinomenine respectively except for control group. 5 weeks after treatment, all rabbits were sacrificed for HE staining and histological grading on cartilago articularis and synovium, and ELISA method was used to detect the contents of MMP-13 and COMP in rabbit serum and synovial fluid.Results Mankin's scores of articular cartilage and histological scores of synovium in model group were significantly higher than those of control group (P<0.01), and were markedly lower in sinomenine group than those of model group (P<0.01); the contents of MMP-13 and COMP in serum and synovial fluid of model group were significantly raised compared with control group (P<0.01), and were reduced obviously in sinomenine group compared with model group (P<0.05,P<0.01).Conclusion Intra-articular injection of sinomenine can reduce the levels of MMP-13 and COMP in serum and synovial fluid of rabbit knee osteoarthritis model, and improve synovial inflammation, as well as delay the degradation of articular cartilage.