The effect of miRNA-29a on the proliferation and apoptosis of HVSMCs cells / 重庆医学

ABSTRACT

Objective To detect the expression of miRNA‐29a in abdominal aortic aneurysm(AAA) patients and construct a recombinant lentiviral vector carrying miRNA‐29a ,transfection of human abdominal aortic vascular smooth muscle cells (HVSMCs) ,and to investigate the alteration on the proliferation in HVSMCs .Methods Collected 25 AAA patients′serum and 25 normal elderly serum specimens from March 2011 to March 2014 ,the mean age were 62 .9 ± 13 .6 and 61 .5 ± 11 .8 separately .Em‐ploy RT‐PCR analysis the miRNA‐29 expression in different group .The MCS‐CMV‐miRNA‐29a was transfected into HVSMCs cells ,the transfection efficiency was observed with fluorescence microscope ,the mRNA and COL1A1 protein expresstion was detec‐ted by RT‐PCR and Western blot respectively .MTS method was used to determine effects of cell inhibition rates of different groups ,cell apoptosis was analysised by Annexin V/7‐AAD double staining after 72 h .Results The expression of miRNA‐29a with AAA was lower than normal group .The transfection efficiency was (74 .25 ± 10 .10)% .miRNA‐29a mRNA and its target protein COL1A1 expression increased significantly compared with the control group after transfection ,MTS and Annexin V/7‐AAD results showed that cell apoptosis was decreased after miRNA‐29a overexpression ,cell proliferation was increased compared with the con‐trol group .Conclusion The miRNA‐29 expression decreased associated with the occurrence of abdominal aortic aneurysm ,but overexpress miRNA‐29a in HVSMC cells by lentivirus mediated ,which could promote the proliferation of cells ,inhibit its apoptosis .