Analysis of anti-gout capsule effect on AGA rats serum, articular cartilage and synovial fluid TNF-αlevel / 中国免疫学杂志

ABSTRACT

Objective:To investigate the anti-gout capsule ( AGC) effect on acute gouty arthritis ( AGA) rat tumor necrosis factor-α(TNF-α)level.Methods: Selected 60 SD rats,were randomly divided into normal group,model group,colchicine group(0.8 mg/kg),AGC low dose group (0.3 g/kg) and AGC high dose group (1.2 g/kg),12 rats in each group,detected each group articular cartilage,serum and synovial fluid TNF-αcontent,observed rats ankle swelling.Results: The model group 24 h,48 h gait behavior scores were(2.57±0.43)point and(2.11±0.50)point,significantly higher than other groups (P0.05 );AGC high dose group and low dose group articular cartilage TNF-αexpression significantly decreased than model group ( P<0.05).The model group serum and synovial fluid TNF-αcontent were significantly higher than the normal group (P<0.05);colchicine group,AGC low dose group and AGC high dose group serum and synovial fluid TNF-αcontent reduced compared with the model group (P<0.05);AGC low dose group and AGC high dose group serum TNF-αwere(0.81±0.27)ng/ml and(0.79±0.31)ng/ml,lower than the colchicines group ( P<0.05 ).Conclusion:AGC can significantly improve the joint symptoms of AGA rats ,decrease AGA rats articular cartilage tissue ,serum and synovial fluid TNF-αlevel.