Neuroprotective effect of neural stem cells modified by glial-derived neurotrophic factor on cerebral apoplexy / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 7331-7336, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-485301
ABSTRACT
BACKGROUND:
Glial-derived neurotrophic factor has a specific effect on brain neurons, and is an important neurotrophic factor in the treatment of cerebral apoplexy.OBJECTIVE:
To investigate the neuroprotective effect of glial-derived neurotrophic factor gene modified neural stem cels on rat cerebral apoplexy.METHODS:
The recombinant human plasmid pAdEasy-l-pAdTrackCMV was constructed, and the neural stem cels were isolated and cultured from the cortex of neonatal rats. The neural stem cels were transfected with the recombinant adenovirus of glial-derived neurotrophic factor, and the cel suspension was injected into the right brain ventricle of rats with transient cerebral ischemia (2 hours). Meanwhile, neural stem cel transplantation group and control group were set up. RESULTS ANDCONCLUSION:
Compared with the neural stem cel transplantation group, the modified neurological severity score of combined transplantation group was reduced significantly 2 and 3 weeks after reperfusion, and the area of cerebral ischemia injury was also significantly decreased at 7 days after reperfusion (P 0.05). The results show that the neural stem cels modified by glial-derived neurotrophic factor can play a better role in the neuroprotection against cerebral apoplexy in rats, and the effect is better than that of simple neural stem cels.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2015
Type:
Article
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