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Plasma Proteomic Analysis of Early Diabetic Nephropathy Patients with Kidney Yang Deficiency / 广州中医药大学学报
Journal of Guangzhou University of Traditional Chinese Medicine ; (6): 890-894, 2014.
Article in Chinese | WPRIM | ID: wpr-485439
ABSTRACT
Objective To screen the sensitive plasma molecular markers in diabetic nephropathy ( DN) patients with kidney yang deficiency. Methods Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to analyze the plasma which was sampled from 4 early DN patients with kidney yang deficiency and 4 healthy adult volunteers. Proteins that showed differential expression with an over 1.5-fold change were analyzed by MALDI-TOF/TOF mass spectrometry. Results Two-dimensional electrophoresis (2-DE) maps of plasma proteins in DN patients with kidney yang deficiency and healthy adults were established successfully. We validated 9 differentially expressed proteins, including complements C3 and C4, apolipoprotein E and ubiquitination factor. Conclusion Proteomic analysis by 2-D DIGE can objectively reveal the differences of plasma protein expression in DN patents with kidney yang deficiency and healthy adult volunteers. The obtained 9 proteins have potential to provide plasma molecular markers for the early clinical diagnosis and for the research of traditional Chinese medical patterns of DN.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Guangzhou University of Traditional Chinese Medicine Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Guangzhou University of Traditional Chinese Medicine Year: 2014 Type: Article