Seminiferous capsule extract effect on the proliferation of spermatogonial stem cells / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 2104-2109, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-486253
ABSTRACT
BACKGROUND:
Chinese herbs for kidney nourishment can promote the proliferation and differentiation of spermatogonial stem cel s.OBJECTIVE:
To verify the effect of seminiferous capsule extract on spermatogonial stem cel proliferation.METHODS:
Spermatogonial stem cel s were isolated from the testis of male mice and synchronized by serum-free medium fol owed by an addition of 10, 50, 100 mg/L seminiferous capsule extracts. After 24 hours of culture, viability, proliferation and cel cycle of spermatogonial stem cel s were observed. RESULTS ANDCONCLUSION:
Compared with the control group, seminiferous capsule extracts promoted the cel number, viability and proportion at S stage. The number of BrdU-labeled spermatogonial stem cel s was increased significantly after intervention with seminiferous capsule extracts, especial y at the concentration of 50 mg/L. These findings indicate that seminiferous capsule extracts can promote the proliferation and viability of spermatogonial stem cel s.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2016
Type:
Article
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