Effect of Ca2+-activated Chloride Channel Density on Gating Properties of Anoctamin 1 / 中国医科大学学报

ABSTRACT

Objective To investigate the effect of channel density on the gating properties of Anoctamin 1(Ano1,TMEM16A)Ca2+?activated chlo?ride channel. Methods Ano1 expression plasmids were transiently transfected into HEK293 cells. High density and low density of Ano1 was ob?tained after expressing the protein for 24 h and 6 h,respectively. Electrophysiological recordings were performed in the whole?cell patch clamp con?figuration. The activation kinetics of current traces was fitted by exponentials. Results The current density was significantly higher in cells express?ing Ano1 for 24 h than those expressing Ano1 for 6 h(P0.05). Conclusion Our findings suggested that chan?nel density regulates the gating of Ano1. High channel density promotes activation of Ano1.