Endothelial-dependent Vasodilatation and Expressions of CyPA, p-ERK1/2 in Experimental Rats With Obesity Combining Atherosclerosis / 中国循环杂志
Chinese Circulation Journal
;
(12): 77-81, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-487005
ABSTRACT
Objective:
To observe the endothelial-dependent vasodilatation and expressions of cyclophilin A (CyPA), phosphorylated extracellular signal regulated kinase1/2 (p-ERK1/2) in experimental rats with obesity combining atherosclerosis.Methods:
A total of 30 male Wistar rats were randomly divided into 3 groupsControl group, the rats received basic diet followed by intraperitoneal injection of normal saline;Atherosclerosis (AS) group, the rats received basic diet for 8 weeks followed by high cholesterol diet with intraperitoneal injection of a single dose vitamin D3 600,000 IU/kg; Obesity+AS group, the rats received high cholesterol diet for 8 weeks (which made their body weights at 20%higher than the other 2 groups) followed by intraperitoneal injection of a single dose vitamin D3 600,000 IU/kg. n=10 in each group. 16 weeks later, the endothelial-dependent vasodilatation was examined in all rats, expressions of CyPA and p-ERK1/2 in arterial wall were detected by HE staining and immunohistochemistry.Results:
Compared with Control group, both AS group and Obesity+AS group had reduced endothelial-dependent vasodilatation (72.49 ± 3.27)%and (42.28 ± 2.62)%vs (96.63 ± 3.85)%, such reduction was even more in Obesity+AS group (42.28 ± 2.62)%vs (72.49 ± 3.27)%, all PConclusion:
The rats with obesity and AS had decreased endothelial-dependent vasodilatation, severe atherosclerosis and calciifcation plaques, increased expressions of CyPA and p-ERK1/2, which speculated that obesity might be an independent risk factor for atherosclerosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Risk factors
Language:
Chinese
Journal:
Chinese Circulation Journal
Year:
2016
Type:
Article
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