Detection of TNF -α,NSE and S100B levels in children with hand -foot -mouth disease complicated enceph-alitis and its clinical significance / 中国基层医药

ABSTRACT

Objective To understand the clinical significance of detection of tumor necrosis factor alpha (TNF -α),neuron specific enolization enzyme (NSE)and S100B levels in children with hand -foot -mouth disease (HFMD)complicated with encephalitis,and to provide research data for children with HFMD complicated with encephalitis.Methods 157 children with HFMD were divided into 79 cases of HFMD complicated encephalitis group and 78 cases of HFMD group according to whether there was encephalitis complications.Another 80 healthy children were chosen as control group.TNF -α,NSE and S100B protein in blood and cerebrospinal fluid of the three groups were detected by enzyme -linked immunosorbent assay.Results TNF -αlevels in blood and cerebrospinal fluid of HFMD complicated encephalitis group,HFMD group were significantly higher than those of the control group (t =8.315,6.443,4.132,6.443,all P <0.05);TNF -αlevels in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of HFMD group (t =4.378,3.220,all P <0.05).NSE in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of the control group (t =8.706,7.840,all P <0.05);NSE in blood and cerebrospinal fluid of HFMD complicated encepha-litis group were significantly higher than those of HFMD group (t =6.945,7.134,all P <0.05).S100B in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of the control group (t =9.443,10.031,all P <0.05);S100B in blood and cerebrospinal fluid of HFMD complicated encephalitis group were significantly higher than those of HFMD group (t =8.552,9.206,all P <0.05).TNF -α,NSE and S100B levels in blood and cerebrospinal fluid of HFMD complicated encephalitis group were positively correlated (r =0.803, 0.794,0.856,all P <0.05);TNF -αlevels in blood and cerebrospinal fluid of HFMD group was positively correla-ted (r =0.743,P <0.05).Conclusion Detection of TNF -α,NSE and S100B levels in blood and cerebrospinal fluid can assist to diagnose children with HFMD,and NSE and S100B can be used as specific indicators of HFMD complicated encephalitis.