Protective effect of Danshen Chuanxiongqin injection on rats with acute kidney injury caused by sepsis / 中国基层医药

ABSTRACT

Objective To discuss the influence of Danshen Chuanxiongqin injection on renal function and relevant indexes of urine in rats with sepsis -induced acute kidney injury(AKI))caused by cecal ligation and punc-ture (CLP).Methods Seventy male Sprague Dawley(SD)rats were randomly divided by random number table into normal group,sham -operation group,sepsis group and Danshen Chuanxiongqin group.The three latter groups were divided into groups of 6,12,24,48 -hour subgroups,five rats in each subgroup.Danshen Chuanxiongqin group was injected with Danshen Chuanxiongqin injection at 0,24,48h from caudal vein seperately,and the other groups were injected with saline solution.The blood and urine were collected in the set four time points.The relevant indexes like serum creatinine (SCr),blood urea nitrogen(BUN),IL -6 and TNF -αwere tested and kidney injury molecule -1 (KIM-1)and neutrophil gelatinase associated lipocalin (NGAL)were detected.Changes of renal pathology were observed under light microscope.Results In the sepsis group,the concentrations of SCr and BUN 6h after CLP star-ted to rise,reached peak in 24h to (96.31 ±12.64)μmol/L and (27.17 ±4.98)mmol/L,and decreased in 48 h, while concentrations of IL -6 and TNF -αwere reached peak in 12h (28.45 ±3.22)pg/mL and 6h (72.35 ± 10.84)pg/mL respectively,and then decreased,which were all higher than those in the normal group and sham -operation group (P <0.05).After Danshen Chuanxiongqin injection therapy,the concentrations of Scr,BUN,IL -6 and TNF -αin 6h,12h,24h and 48 h were all lower than those in the corresponding points in time in the sepsis mod-el group,and reached minimum values[(14.89 ±2.41)μmol/L,(17.29 ±3.37)mmol/L,(14.89 ±2.41)pg/mL, (17.29 ±3.37)pg/mL,all P <0.05].The contents of KIM-1 and NGAL 6 h after CLP started to increase,reached peak in 24h (5.47 ±0.39)ng/mL and 12h (6.35 ±0.81)ng/mL respectively,and decreased in the sepsis group were all remarkably higher than those in the normal group and sham -operation group.After given Danshen Chuanx-iongqin injection,the contents of KIM-1 and NGAL in 6h,12h,24h and 48h were greatly lower than those in the corresponding points in time in the sepsis group,reached minimum values[(2.27 ±0.16)ng/mL,(2.03 ±0.27)ng/mL,all P <0.05)].In the Danshen Chuanxiongqin group,the change of renal pathology was better than that of the sepsis group in 12h.Conclusion Danshen Chuanxiongqin injection plays the role of renal protection in AKI caused by sepsis.