Determination of Mahuannin A in Ephedrae Radix et Rhizoma by HPLC-ELSD / 中国药师
China Pharmacist
;
(12): 360-361, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-487039
ABSTRACT
Objective:
To establish an HPLC-ELSD method for the determination of mahuannin A in ephedrae radix et rhizoma.Methods:
The content of mahuannin A was determined by an HPLC-ELSD method on an Alltima TM C18 column (250 mm × 4. 6 mm, 5 μm). The mobile phase was acetonitrile-water (28∶ 72) with a flow rate of 0. 7 ml·min-1, and the column temperature was 30℃. The temperature of drift tube heater was 105℃ and the flow rate of carrier gas was 2. 8 L·min-1 .Results:
The linear range of mahua-nnin A was 42. 56-383. 04 μg·ml-1(r=0. 999 8). The average recovery and RSD was 99. 9% and 1. 96%(n=6), respectively.Conclusion:
The method is simple and the result is accurate. It can be used for the quality control of ephedrae radix et rhizaoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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