Correlation of regulatory B cells and regulatory T cells in patients with chronic hepatitis C / 中国基层医药

ABSTRACT

Objective To investigate expression and correlation of IL -10 +CD +19 regulatory B cells (Breg)and CD +4CD25high regulatory T cells (Treg)in patients with chronic hepatitis C.Methods 45 patients with chronic hepatitis C and 25 healthy individuals were involved in this study.Flow cytometry was used to analyze the expression of Breg and Treg.ELISA was employed to determine serum IL -10 and TGF -β.Results The expression of Breg in group of HCV was higher than that of the health controls[3.55%(2.40% -4.14%)vs.1.66%(1.24% -2.02%),t =6.78,P <0.001],and Treg in patients with chronic hepatitis C was also higher than that of the health controls[4.55%(3.35% -5.88%)vs.2.05%(1.39% -2.61%),t =7.17,P <0.001].Breg was correlated with Treg positively in group of chronic hepatitis C (r =0.374,P =0.019)and no correlation was found in the health con-trols.Serum IL -10 in patients infected with HCV was significantly higher than that in the health controls[(78.65 ± 17.21)pg/mL vs.(53.21 ±11.47)pg/mL,t =7.51,P <0.001].Conclusion Breg and Treg may play regulatory roles by IL -10 in patients with chronic hepatitis C promote persistence of HCV infection and exhaustion of effector T cells.