Relationship between the expression of long-non-coding RNA ( lncRNA ) HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma / 中华放射肿瘤学杂志

ABSTRACT

Objective To investigate the relationship between the expression level of HOTAIR and cellular radiosensitivity in esophageal squamous cell carcinoma (ESCC). Methods Four ESCC cell lines ( K150, K450, TE-1, and Eca109 ) were used in this study. Quantitative real-time polymerase chain reaction was applied to measure the expression level of HOTAIR in the above cell lines;colony-forming assay was applied to measure the survival fraction of different cells irradiated by different doses of X-ray. The t-test or analysis of variance was applied for analysis of differences. The correlation analysis was used by Pearson methods. Results The four cell lines all showed high expression levels of HOTAIR and radioresistance. Compared with the other three cell lines, Eca109 had a lower expression level of HOTAIR, a lower survival fraction at each radiation dose point, and significantly lower D0 and Dq . The mRNA expression level of HOTAIR and radiosensitivity were K150analysis of the expression level of HOTAIR and D0/Dq revealed that the expression level of HOTAIR was positively correlated with SF2 and D0(r=0.643 and 0.777,respectively,both P<0.05). Conclusions There is a significant correlation between the expression level of HOTAIR and cellular radiosensitivity, and the expression level of HOTAIR may be an indicator to predict cellular radiosensitivity.