Analysis on correlation between cytotoxic T lymphocyte antigen 4 gene polymorphisms and susceptibility to hepatocellular carcinoma / 重庆医学

ABSTRACT

Objective To analyse the correlation between cytotoxic T-lymphocyte antigen-4(CTLA-4)gene polymorphism-318 T/C ,CT60 G/A and the susceptibility of hepatocellular carcinoma (HCC) .Methods Totally 277 cases of HCC and 306 healthy controls in our hospital from September 2012 to September 2014 were selected as the research subjects ,all subjects were Han nationality .The peripheral blood (5 mL) in each case was collected for separating serum ,the AFP level was detected by chemilunescent method ,the serum IL-2 ,IL-4 and TGF-β levels were detected by ELISA .Genomic DNA was extracted ,after PCR amplification ,the CTLA-4-318 T/C ,CT60 G/A gene polymorphism distributions were detected by the direct sequencing method . Results The CTLA-4-318 CC and CT60 AA genotypes all conformed to the Hardy-Weinberg equilibrium(P> 0 .05) .CTLA-4-318 CC and CT60 AA were related with the HCC risk decrease ,different genotypes of CTLA-4 CT60 G/A had obvious relation with serum AFP level(χ2 =12 .779 ,P=0 .012) .The serum IL-2 and IL-4 levels in the HCC patients carrying CTLA-4-318 T and CT60 G allele were significantly decreased ,while the TGF-βlevel was significantly increased ,moreover CTLA-4-318 had obvious relation with the HCC grading .Conclusion CTLA-4-318 TT could promote the occurrence and progression of HCC ,which might be related with the down-regulation of Th1/Th2 type cytokines ,and up-regulation of Th3 type cytokine .