Separation and Enrichment of Antibacterial Effective Fraction of Syringae Folium by Macroporous Resins / 中国药师
China Pharmacist
;
(12): 437-439, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-487718
ABSTRACT
Objective:
To investigate the purification of antibacterial effective fraction of Syringae folium by macroporous resins.Methods:
Static adsorption and desorption tests were carried out to screen the macroporous resins. The desorption experiment was per-formed on the selected D101 resin to optimize the separation process. The effects of resin amount, diameter length ratio, elution flow rate, elution solution concentration and volume were studied.Results:
The optimal conditions were as followsthe elution solution was 55% ethanol, the adsorption flow rate was 1 BV·h-1 , the elution flow rate was 5 BV·h-1 , 6 BV 25% ethanol was used to eliminate impurity and 8 BV 55% ethanol was used to elute to obtain the effective fraction.Conclusion:
The content of antibacterial effective component is above 65% after purified by D101 resin, indicating that the present method is suitable for large-scale preparation of anti-bacterial effective fraction of Syringae folium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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