Influence of NADH Concetration in the AST Reagent on Assay Performance / 现代检验医学杂志

ABSTRACT

Objective To estimate the assay performance of two different kind of reagent with different concentration of NADH.Methods Verificated function index of these two reagents,such as accuracy,sensitiveness,blank space and so on, and determined the molar extinction coefficient of NADH using hexokinase method and calculated the NADH density in two kind of AST reagents.Then estimated the effect of concentation of NADH on the analysis performance.Results NADH concentration was 0.21 mmol/L,and the sample detection limit of the linear was 1 629 U/L.NADH concentration was 0.13 mmol/L,and the sample detection limit of the linear was 1 263 U/L.So the gap between the two reagent’s detection linear was visible.But there were no significant changes in other performance indicators such as detection sensitivity,reagent blank,precision.Conclusion The NADH concentration in the reagent of AST had greatimpact on the detecting linear range, so should pay attention to the potential change of linear range in the daily work.