Effect of propofol on microcirculation in a rabbit model of hemorrhagic shock and resuscitation / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of propofol on microcirculation in a rabbit model of hemorrhagic shock and resuscitation.Methods Twenty-four adult male rabbits,weighing 1.7-2.1 kg,aged 4-6 months,were equally randomized into sham operation (group S),hemorrhagic shock and resuscitation (group HS/R),or propofol (group P) groups.Hemorrhagic shock was induced by withdrawing blood from the left common carotid artery until mean arterial pressure was reduced to 40-50 mmHg which was maintained for 30 min.The animals were then resuscitated by infusion of shed blood and normal saline (2 times the volume of shed blood) in HS/R and P groups,and in addition propofol 20 mg · kg-1 · h-1 was infused intravenously in group P.Before blood-letting,immediately before resuscitation,and at 30 and 60 min after onset of resuscitation,arterial blood samples were collected for blood gas analysis,and pH value and lactic acid concentrations were recorded.Side-stream dark field imaging was employed to acquire the images of mesenteric microcirculation.Microcirculatory parameters like total vessel density,perfused vessel density,proportion of perfused vessels,and microvascular flow index were recorded.Results Compared with group S,pH value was significantly decreased,and lactic acid concentrations were increased immediately before resuscitation,and at 30 and 60 min after onset of resuscitation,and the total vessel density,perfused vessel density,proportion of perfused vessels,and microvascular flow index were decreased immediately before resuscitation in HS/R and P groups (P＜0.05).There was no significant difference in the parameters mentioned above between group HS/R and group P (P＞0.05).Conclusion Propofol exerts no effect on microcirculation in a rabbit model of hemorrhagic shock and resuscitation.