Association of expressions and gene polymorphisms of leptin receptor with breast cancer / 肿瘤研究与临床

ABSTRACT

Objective To evaluate the association of expressions and gene polymorphism of leptin receptor (LEPR) in breast cancer with tumorigenesis,development and clinicopathologic factors.Methods The immunohistochemical method and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were used to evaluate LEPR expressions and LEPR Gln223Arg polymorphism in 150 cases with breast cancer,80 cases with benign breast lesions,50 cases with corresponding normal breast tissue and 128 healthy controls.Results The expression rate of LEPR genes in breast cancer tissues was significantly higher than that in benign breast lesions and that in corresponding normal breast tissues [70.67 ％ (106/150) vs 56.25 ％ (45/80) vs 44.00 ％ (22/50),P ＜ 0.005].In breast cancer patients,LEPR gene Gin223Arg genotype polymorphism (GG,GA and AA) frequencies were 70.00 ％ (105 cases),16.67 ％ (25 cases) and 13.33 ％ (20 cases),which were significantly different from those in the benign breast lesions [82.50 ％ (66 cases),13.75 ％ (11 cases),3.75 ％ (3 cases)],those in corresponding normal breast tissues [82.00 ％ (41 cases),14.00 ％ (7 cases),4.00 ％ (2 cases)] or those in the health controls [82.81％ (106 cases),14.85 ％ (19 cases) and 2.34 ％ (3 cases)] (X2 =11.56,P =0.003),while the differences of GG,GA and AA genotype requencies among the breast benign disease group,cancer adjacent normal breast group and healthy control group were not statistically significant (P ＞ 0.05).The frequencies of alleles genes in breast cancer patients (G and A) were 78.33 ％ (235 cases) and 21.67 ％ (65 cases),and the differences were statistically significant compared with those in the benign disease group or in healthy control group (X2 =12.52,P =0.001).The positive expression rate of LEPR gene in patients with lymph node metastasis was 87.8 ％,which was higher than that in patients with no lymph node metastasis (60.2 ％) (P =0.02).According to the results of multivariable analyses,high expression of LEPR gene,LEPR Gin223Arg gene polymorphisms and increased waist-hip ratio (WHR) were risk factors for breast cancer (all OR ＞ 1).Conclusion High expression of LEPR,LEPR Gln233Arg polymorphism and the elevated WHR may be correlated with breast cancer.