Identification and phylogenetic relationship of dermatophytes based on RFLP analysis and nucleotide sequence of internal transcribed spacer (ITS)1 in nuclear ribosome DNA
Journal of the Korean Society for Microbiology
;
: 49-60, 2000.
Article
in Korean
| WPRIM
| ID: wpr-48976
ABSTRACT
ITSI-5.8S-ITSII rDNA region was amplified from the reference strains and clinical isolates with ITS1 and ITS4 primers. These primers amplified DNA fragments of 550 bp in Microsporum audouinii and Trichophyton violaceum, 700 bp in Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum, and Trichophyton tonsurans, and 750 bp in Microsporum ferreugineum and Microsporum canis. The restriction enzyme patterns of PCR products digested with 13 restriction enzyme including PstI were distint among the genera, whereas identical in the same species. Examination of the ITS (Internal Transcribed Spacers)1 nucleotide sequence revealed that there was the genetic difference in each genera and species. Phylogenetic relationship among each species showed that the Trichophyton mentagrophytes was more closely related Trichophyton tonsurans than Trichophyton rubrum, and Microsporum gypseum was less related than Microsporum spp.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ribosomes
/
Trichophyton
/
Polymorphism, Restriction Fragment Length
/
DNA
/
DNA, Ribosomal
/
Base Sequence
/
Polymerase Chain Reaction
/
Arthrodermataceae
/
Microsporum
Type of study:
Diagnostic study
Language:
Korean
Journal:
Journal of the Korean Society for Microbiology
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS