Biocompatibility of hirudin peptide-modified acrylic intraocular lens / 中国组织工程研究

ABSTRACT

BACKGROUND:Because of poor biocompatibility, conventional intraocular lenses (IOLs) used to improve visual acuity can result in a higher incidence of inflammatory foreign body reactions. OBJECTIVE:To study the biocompatibility and therapeutic effects of hirudin peptide-modified acrylic IOL on eye diseases. METHODS:By immersion coating method, we prepared a polymethylmethacrylate IOL by the modification with hirudin polypeptides and observed its biocompatibility through the cytological observation. In this study, six New Zealand white rabbits were equivalently randomized into conventional and hirudin peptide grouppolypeptide hirudin group. Rabbits in the conventional group were given conventional lens implantation; while those in the hirudin peptide group underwent hirudin peptide-modified polymethylmethacrylate IOL. RESULTS AND CONCLUSION:Three days after implantation of hirudin peptide-modified polymethylmethacrylate IOL, cels proliferated and spread wel on the acrylic IOL, but on the cel membrane, there were a few cels most of which were gathered sphericaly with no spreading. In the hirudin peptide group, some cels attached to the border zone migrated to the IOL surface, but did not impede the central visual field; in the conventional group, the IOL surface was stil covered by fibrotic cel membrane.Comparedwith the conventional group, the score in the SchirmerItest was increased but the breakup time of tear film decreased in the hirudin peptide group. To conclude, the polymethylmethacrylate IOL modified by hirudin peptides has good physicochemical properties and biocompatibility, increases visual acuity, and yields satisfactory outcomes in the cataract treatment, which provides a new insight into the treatment of cataract.