Effects of combined treatment using umbilical cord blood mesenchymal stem cell transplantation and paclitaxel on proliferation, apoptosis, and invasion of A2780 ovarian cancer cells / 中国组织工程研究

ABSTRACT

BACKGROUND:An intensivestudy on ovarian cancer A2780 cels contributes to modify treatment strategies targeting ovarian cancer stem cels and enhance survival rate of ovarian cancer patients. OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer celsin vitro. METHODS:A2780 human ovarian cancer cels were assigned into three groups blank control group (routine cel culture),UC-MSCs group (addition of suspension containing 1×106UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106UC-MSCs and 250μL of 1μmol/L paclitaxel solution). CD133+antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwel chamber invasion assay, respectively. RESULTS AND CONCLUSONCD133+antigen expression intheUC-MSCs group and combined treatment group was significantly decreased compared withtheblank control group (P< 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cel number was significantly increased inthecombined treatment group compared with the other two groups (P< 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosisof A2780 human ovarian cancer cels.