Biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect / 中国组织工程研究

ABSTRACT

BACKGROUND:Articular cartilage regeneration can be regulatedbyautocrineorparacrinesecretionof various cytokines. OBJECTIVE:To analyze biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect. METHODS:Forty New Zealand white rabbits were used and equaly randomized intofourgroups fibrin, basic fibroblast growth factor, bone morphogenetic protein, and combined treatment (basic fibroblast growth factor combined with bone morphogenetic protein) groups, respectively.Bioactivescaffolds with fibrin, basic fibroblast growth factor,bone morphogenetic protein, and basic fibroblast growth factor combined with bone morphogenetic protein were injected to repair the articular cartilage defect. Therapeutic effect andbiological properties of biological materials were compared. RESULTS AND CONCLUSION:(1) Inthefibrin group,tworabbits appearedto havelimps. Inthebasic fibroblast growth factor group hand functionwaslimited inonerabbit. Inthebone morphogenetic protein group, one had a limpandonewasin a limitation of activity. Inthecombined treatment group,rabbitsrecovered wel andshowedno differencesintheknee joint before and aftersurgery (P< 0.05). (2) General observation Inthe combined treatment group, soft solution cartilage defects disappeared,andangiogenesis and cartilage were similar with normal tissues. Inthebone morphogenetic protein group, fractured cartilage marginal existedand could not beclosely integrated with normal cartilage. The presence of chondrocytesin the periphery of the defectwas seen under light microscope. Inthefibrin group, defect site and surrounding tissues healed. Inthe basic fibroblast growth factor group, defect was repaired, but not smooth. (3) Results ofhematoxylin and eosin staining Inthefibrin group, the bone defect was not repaired, obvious depression surface was seen. In basic fibroblast growth factor group, repair of cartilage defect was obvious. There were a lot ofchondrocytes. Inthe bone morphogenetic protein group, the bone defect was repaired; chondrocytes appeared, but irregular arrangement. Inthecombined treatment group, good bone defect repair and a large number of cartilage cels were seen. Taken together, biological materials with fibrin and basic fibroblast growth factor are ideal for repair of articular cartilage defect by promoting formation of cartilage by bone morphogenetic protein and enhancing chondrocyte proliferation by basic fibroblast growth factor.