Determination of Baicalin and Geniposide in Ganxueguanliu Huoxue Capsules by HPLC / 中国药师
China Pharmacist
;
(12): 772-774, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-490873
ABSTRACT
Objective:
To build an HPLC method for the determination of baicalin and geniposide in Ganxueguanliu Huoxue cap-sules.Methods:
An Agilent Eclipse XDB-C18 (150 mm × 4. 6 mm, 5μm) column was chosen for the analysis. The mobile phase con-sisted of 0. 2% phosphoric acid (A) and methanol (B) with gradient elution. The flow rate was 1. 0 ml·min-1. The detection wave-length was set at 238 nm and the column temperature was 25℃.Results:
A good linear relationship was observed within the range of 0. 418-8. 360 μg·ml-1 for baicalin (r=0. 999 9) and 0. 226-4. 524μg·ml-1 for geniposide (r=0. 999 9). The average recovery of baicalin and geniposide was 99. 79% (RSD=1. 55%, n=6) and 100. 2%(RSD=2. 05%, n=6), respectively.Conclusion:
The method is rapid, convenient and specific, which can be used for the content determination of baicalin and geniposide in Ganxueguanliu Huoxue capsules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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