Protective Effect of Curcumin on Intestinal Epithelial Cell Barrier Disruption / 胃肠病学

ABSTRACT

Background:Disruption of intestinal epithelial tight junction and the followed barrier function play important roles in the pathogenesis of intestinal disorders. Curcumin could provide protection for the impaired barrier function. Aims:To investigate the protective effect of curcumin on ethanol-induced intestinal mucosal barrier disruption. Methods:Caco-2 cells were cultured to establish intestinal epithelial cell barrier model in vitro,and then were divided into control group, ethanol group and different concentrations of curcumin groups(5,20,80 μmol/ L curcumin). Trans-epithelial electrical resistance(TEER)and flux of sodium fluorescein for Caco-2 cell monolayers were measured to examine intestinal epithelial barrier function. Expression and localization of Occludin protein were measured by Western blotting and immunofluorescence,respectively. Cell structure was observed by transmission electron microscopy( TEM). Results:Compared with control group,TEER was significantly decreased and flux of sodium fluorescein was significantly increased (P < 0. 05),expression of Occludin protein was significantly decreased(P < 0. 05)in ethanol group. Immunofluorescence showed that Occludin protein expression was discontinuous and fluorescence intensity was low. TEM showed that brusher border was disorganized,and cell-cell junction was vague. When pretreated with curcumin,the above-mentioned indices were significantly improved,especially in 20 μmol/ L curcumin group( P < 0. 05). Conclusions:Curcumin protects ethanol-induced intestinal epithelial cell barrier disruption.