Effect of protoscolex on T subsets in mice spleen cells in vitro / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 174-177, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-491615
ABSTRACT
Objective:
To observe the effect of protoscolex on Th subsets and correlative cytokine in mice spleen cells in vitro.Methods:
Co-culture spleen cells from BALB/c mice with protoscolices,then IL-4,IFN-γand TGF-βproduction in cell culture supernatants were analyzed by ELISA.The percentage of Th subsets were detected by Flow Cytometry analysis.Results:
Secretion levels of IL-4 and TGF-βwere significantly increased in spleen cells at different time point in co-culture system with protoscolices.Ratios of Th2 and Treg cells were also significantly increased in co-culture system at different time points than the control groups.However,there was no statistical significance for ratio of Th1 cells at different time points.Conclusion:
The protoscolex can increase the ratios of Th2 cells and Treg cells from spleen cells.Secretion levels of IL-4 and TGF-βwere also increased in spleen cells co-cultured with protosco-lices.The results suggest that these Th cell subsets play a role in the immune escape of the hydatid disease.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2016
Type:
Article
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