Autologous endothelial progenitor cells promote the neovascularization of tissue engineering bone in vitro and vivo / 重庆医学

ABSTRACT

Objective Investigate the ability of autologous endothelial progenitor cells (EPCs) in promoting the neovascular-ization of tissue engineering bone in vitro and vivo .Methods Co-culture EPCs drived from autologous peripheral blood and mesen-chymal stem cells drived from bone marrow (BMSCs) at the best proportion of 1 ∶ 2 which was the largest cell proliferation rate in vitro ,osteogenesis related cytokines Osteonectin ,Osteopotin ,Collagen Type 1(Col-1) and angiogenesis related cytokine VEGF in vitro by using real-time quantitative polymerase chain reaction(PCR) ,and compared with pure EPCs and BMSCs groups at 3rd ,7th and 14th day ;the tissue engineering bone seeded with EPCs ,BMSCs and co-culture cells (EPCs ∶ BMSCs = 1 ∶ 2) were transplanted into rabbit limbs muscle ,the growing states of tissue engineering bone were observed at 2 ,4 and 8 weeks after transplantation ,at the same time the expression of CD34 ,CD105 and ZO-1 were detected with immunohistochemistry staining .Results The mRNA expression of Osteonectin ,Osteopotin ,Col-1 and VEGF were gradually increased when detected at 3rd ,7th and 14th day with real-time PCR ,and the co-culture cells group increased most obviously in the three groups in vitro at the same period time(P< 0 .01) ;The microvascularization of the engineering biological bone were observed in vivo with immunohistochemistry ,and neovasculariza-tion of co-culture cells group was also the most obvious group in three groups ,immunohistochemical showed that CD34 ,CD105 and ZO-1 was also higher than the other two groups(P< 0 .01) .Conclusion Autologous EPCs interact with BMSCs could promote vas-cularization of tissue engineering bone both in vivo and in vitro .