Effect and mechanism of simvastatin on proliferation and cycle of glomerular mesangial cells cultured in high-concentration glucose / 重庆医学

ABSTRACT

Objective To study the effect of simvastatin(SIM ) on the proliferation and cycle of glomerular mesangial cells (GMCs) cultured in high concentration of glucose ,and to observe the variation of inflammation factor and extracellular matrix . Methods GMCs of rats were divided into 4 groupslow glucose(LG) group(Glu 5 .6 mmol/L) ,High glucose (HG) group(Glu 30 mmol/L) ,HG + SIM (10 μg/L)group and HG + SIM group(25 μg/L) .Cells′ proliferation activity was tested by M TT ,simultane-ously compared the ratio of cells in different period (G0 /G1 ,G2 + M and M ) ,and levels of TGF-β1 ,FN and COL4 were compared a-mong different groups .Results Absorbancy (A) of different periods in HG group was higher than that in other 3 groups ,difference had statistical significance(P< 0 .05) .In LG group ,the ratio of cells in G0 /G1 were(35 .67 ± 3 .38)% while S-stage were(41 .24 ± 5 .64)% ;in HG group ,the ratio of cells in G0 /G1 were(25 .88 ± 4 .02)% while S-stage were(28 .65 ± 1 .88)% ;in HG + SIM (10 μg/L)group ,the ratio of cells in G0 /G1 were(28 .12 ± 2 .01)% while S-stage cell were(35 .55 ± 2 .09)% ;in HG + SIM (25 μg/L ) group ,the ratio of cells in G0 /G1 were(31 .85 ± 3 .52)% ,while in S-stage were(39 .82 ± 2 .23)% ,the difference had statistical sig-nificance(P< 0 .05) .Levels of TGF-β1 ,FN and COL4 in HG group were higher than those of in HG + SIM (10 μg/L)group ,HG +SIM (25 μg/L)group and LG group ,the difference had statistical significance(P< 0 .05) .Conclusion By decreasing TGF-β1 level , simvastatin can inhibit the proliferation of GMCs and regulate the cell cycle ,to suppress the hypertrophy of GMCs .