Clinical distribution of Hemophilus influenzae and its drug resistance analysis / 国际检验医学杂志

ABSTRACT

Objective To study the distribution of clinically separated Hemophilus (H .) influenzae and its drug resistance situa‐tion .Methods Among 2 663 specimens of screening qualified and positive culture collected from the outpatients ,inpatients and e‐mergency patients of our hospital from January to December 2014 ,including 1 895 specimens of lower respiratory tract ,666 blood specimens and 102 urinary secretion specimens ,438 strains of H .influenzae were isolated .The cephalosporin nitro thiophene paper test strip was used to test its beta lactamase and the K‐B method was used to detect its sensitivity to 8 kinds of antibacterial drugs . The testing data were statistically analyzed by using WHONET 5 .6 software .Results Among 438 strains of H .influenzae ,98 .6%was originated from hospitalized patients ,1 .4% from the outpatient department ;176 isolated strains were originated from children , accounting for 40 .18% ;194 strains (44 .29% ) were from elderly patients ,434 strains(99 .09% ) were originated from the lower re‐spiratory tract specimens .The resistance rate to compound sulfamethoxazole was highest and reached 82 .90% ,which to ampicillin and tetracycline were 29 .00% and 43 .40% respectively ;but which to cefuroxime ,ceftriaxone ,ciprofloxacin ,azithromycin ,imipenem were below 2 .00% .The β‐lactamase positive rate was 43 .40% ,in ampicillin resistant strains ,1 .57% of strain was negative in β‐lac‐tamase test .The resistance to ampicillin and enzyme production rate had no significant differences between the children isolated strains and adult isolated strains (42 .43% ,42 .40% vs .44 .00% ,44 .00% ) .Conclusion H .influenzae is mainly isolated from low ‐er respiratory tract specimens ;which is dominated by children and elderly patients ;the second and third generations of cephalospo‐rins and azithromycin can serve as a clinical preferred drugs ;β‐lactamase is the primary mechanism of resistance to ampicillin .