Immunoprotection of gastric mucosa in newborn mice after oral immunization with inactived H.pylori / 中国免疫学杂志

ABSTRACT

Objective:To explore the Immunoprotection of gastric mucosa in newborn mice after oral immunization with inactived H.pylori.Methods:Thirty-two BALB/c mice were randomly divided into four groups, including PBS control group ( groupⅠ) ,the group of inactivated H.pylori ( groupⅡ) , the group of inactivated H.pylori and Escherichia cole heat-labile enterotoxin B subunit( LTB) ( groupⅢ) ,and normal group( groupⅣ).Newborn BLAB/c mice within 24 hours were immunized through oral gavage with corresponding antigen,Two weeks after final immunization,the mice were challenged with 1×108CFU live H.pylori strain SS1, twice once day,for 2 days.The twenty-eighth day post-challenge,all of mice were sacrificed and stomachs were removed for grades of in-flammation and The counting of H.pylori colonizing.Results:The mean scores of stomach tissue inflammation of groupⅠ,Ⅱ,Ⅲ andⅣwere 2.25±0.46,2.12±0.35,1.5±0.53,0.25±0.46,respectively,and the score of inflammatory activity of the above four groups were 2.12±0.35,2.12±0.35,1.37±0.52,0.25±0.46.Among these four groups,the results were statistically significant between any two groups except the result between groupⅠand groupⅡ.Finally,H.pylori colonization quantity in the stomachs of groupⅢwas lower than groupⅠand group Ⅱ( P<0.05 ) , as expected, the H.pylori colonization was undetectable in group Ⅳ.Conclusion: The oral gavage with inactived H.pylori plus LTB to newborn mice may be associate with the lightening of inflammation and the decrease of H.pylori colonizing number.