Effects of detoxificating and stasis-removing herbal medicament on immune function in immunosuppressive mice / 国际药学研究杂志

ABSTRACT

Objective To study the regulative effects of herbal medicament on the immune functions of immunosuppressive?model mice. Methods The dexamethasone induced immunosuppressive mice model was adopted. Comparison was made with the normal control group(sterile water)and the positive control group(levamisole). In order to identify the role of the immune function of differ?ent doses of the herbal medicament on proliferation degree of T and B lymphocyte as well as the influence on the NK cells activity in vivo and in vitro were tested. The low,medium and high dose were 2.4,4.8 and 7.2 g/kg respectively in vivo,and 25,50 and 100 mg/L respectively in vitro. Results The herbal medicament had obvious proliferation effect on T lymphocyte of immunosuppressive mice model induced by dexamethasone with or without ConA stimulation in vivo and in vitro(P<0.01). Proliferation function was also shown on B lymphocyte of mice with or without stimulation of LPS in vivo and in vitro(P<0.01). It had no proliferation effect on NK cells in vivo and in vitro. Under the condition with or without interleukin stimulating NK cell killing rate was all increased. Conclu?sion The herbal medicament improves the immune function of low immunity mice mainly based on proliferation of T and B lym?phocytes and increased lethality of NK cells.