Application of Two Kinds of Tissue Clearing Method in Observing 3D Imaging of Spinal Cord with Immunofluo-rescent Staining / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 417-421, 2016.
Article
in Zh
| WPRIM
| ID: wpr-492203
Responsible library:
WPRO
ABSTRACT
Objective To compare the application of CUBIC and iDISCO clearing methods in observing 3D imaging of spinal cord with immunofluorescent staining. Methods 1 mm thick spinal cord coronal sections were processed with CUBIC and iDISCO, respectively. The neurofilament (NF) protein was detected by immunofluorescent staining and then was observed by a laser confocal microscope. Results Compared with CUBIC, iDISCO had the advantages of shorter time, higher transparency (F=6.64, P<0.01), and deeper penetration (F=5117.55, P<0.01). Conclusion Immunofluorescent staining combined with iDISCO could completely observe the spinal axons with shorter time and better stain effect.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Rehabilitation Theory and Practice
Year:
2016
Type:
Article