Effects of miR-506 on malignance phenotypes of hepatocellular carcinoma cells / 天津医药
Tianjin Medical Journal
; (12): 552-555, 2016.
Article
in Zh
| WPRIM
| ID: wpr-492375
Responsible library:
WPRO
ABSTRACT
Objective To investigate effects of microRNA-506 (miR-506) on malignant phenotypes of hepatocellular carcinoma (HCC) cells, including cellular viability, proliferation and invasion. Methods HCC cell lines HepG2 and QGY-7703 were served as model. Five experimental groups were established in this study, including cell control, pcDNA 3 blank vector control, miR-506 over-expression, pSIH1 blank vector control and miR-506 suppression groups. Real-time reverse transcription PCR assay was performed to measure miR-506 level. CCK-8, colony formation and Transwell assays were performed to detect viability, colony formation activity and invasion activity of HCC cell lines, respectively. Effects of miR-506 on these indexes were evaluated. Results In HepG2 and QGY-7703 cell lines, miR-506 level increased in the miR-506 over-expression group (P0.05). Conclusion miR-506 plays a tumor suppressor role in HCC cells by inhibiting cell viability, colony formation and invasion.
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WPRIM
Language:
Zh
Journal:
Tianjin Medical Journal
Year:
2016
Type:
Article