Oligodendrocyte transplantation for acute spinal cord injury：insulin-like growth factor 1 induces bone marrow mesenchymal stem cells differentiating into oligodendrocytes / 中国组织工程研究

ABSTRACT

BACKGROUND:Treatment and rehabilitation of spinal cord injury is a complicated problem,andthe reconstruction and remyelination of neural reflex pathwaysare the essentialprocess, during which oligodendrocytes play an important role in spinal cord injury repair. OBJECTIVE:To observe theeffect ofoligodendrocyte transplantation for acute spinal cord injury in rats. METHODS:Insulin-like growthfactor 1 induced bone marrow mesenchymal stem cels differentiating into oligodendrocytes, and those oligodendrocytes were transplanted into rats with acute spinal cord injury as induced cel transplantation group. Simple normal salineandnatural oligodendrocytes were transplanted into the rat injured spinal cord as control group and oligodendrocyte group, respectively. Rat behavioral changes were observed by inclined planetest and Basso-Beattie-Bresnahan (BBB) scores. Neurological recovery and survivalof the transplanted cels was detected and observed using spinal evoked potential and immunohistochemical staining, respectively. RESULTS AND CONCLUSION:Compared with the control group, BBB scores and the criticalelevation angle oftheincline planetestsignificantly increased, latencies of spinal motor and sensory evoked potential were on the decline (P< 0.05), and there were no significant differencesin above indicators between the two groups at 4 and 8 weeks after transplantation. Moreover, survivedoligodendrocytes after transplantation could be found in the lesions of spinal cord in both two groups. In conclusion, insulin-like growth factor 1-induced bone marrow mesenchymal stem cels can differentiate into oligodendrocytesthat exact an excelentrole in acute spinal cord injury repair after transplantation, which achieve the equal clinical efficacy tothenatural oligodendrocytes.