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Modulation of the caveolin-3 localization to caveolae and STAT3 to mitochondria by catecholamine-induced cardiac hypertrophy in H9c2 cardiomyoblasts
Experimental & Molecular Medicine ; : 226-235, 2009.
Article in English | WPRIM | ID: wpr-49345
ABSTRACT
We investigated the effect of phenylephrine (PE)- and isoproterenol (ISO)-induced cardiac hypertrophy on subcellular localization and expression of caveolin-3 and STAT3 in H9c2 cardiomyoblast cells. Caveolin-3 localization to plasma membrane was attenuated and localization of caveolin-3 to caveolae in the plasma membrane was 24.3% reduced by the catecholamine-induced hypertrophy. STAT3 and phospho-STAT3 were up-regulated but verapamil and cyclosporin A synergistically decreased the STAT3 and phospho-STAT3 levels in PE- and ISO-induced hypertrophic cells. Both expression and activation of STAT3 were increased in the nucleus by the hypertrophy. Immunofluorescence analysis revealed that the catecholamine-induced hypertrophy promoted nuclear localization of pY705-STAT3. Of interest, phosphorylation of pS727-STAT3 in mitochondria was significantly reduced by catecholamine-induced hypertrophy. In addition, mitochondrial complexes II and III were greatly down-regulated in the hypertrophic cells. Our data suggest that the alterations in nuclear and mitochondrial activation of STAT3 and caveolae localization of caveolin-3 are related to the development of the catecholamine-induced cardiac hypertrophy.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Catecholamines / Cell Line / Caveolae / Myocytes, Cardiac / STAT3 Transcription Factor / Caveolin 3 / Hypertrophy / Mitochondria / Myocardium Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Catecholamines / Cell Line / Caveolae / Myocytes, Cardiac / STAT3 Transcription Factor / Caveolin 3 / Hypertrophy / Mitochondria / Myocardium Limits: Animals Language: English Journal: Experimental & Molecular Medicine Year: 2009 Type: Article