Uncertainty Evaluation of the Determination of Lamotrigine in Human Plasma by LC-MS/MS / 中国药师
China Pharmacist
;
(12): 854-858, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-493918
ABSTRACT
Objective:
To evaluate the uncertainty in the determination of lamotrigine(LTG)in human plasma by LC-MS/ MS.Methods:
The uncertainty sources in the determination of LTG were analyzed and the uncertainty was evaluated and combined. Re-sultsThe expanded uncertainty of LTG at low(0. 050 4 μg· ml - 1 )and high(1. 27 μg· ml - 1 )concentrations was 0. 005 18 μg· ml - 1 and 0. 066 4 μg· ml - 1 ,respectively(P = 95% ,k = 2).Conclusion:
The uncertainty in the determination of LTG in human plasma by LC-MS/ MS is mainly caused by the protein precipitation recovery,matrix effect and sample preparation at low concentration, and by the matrix effect,sample preparation and repeatability at high concentration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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