Fibrin glue combined with vascular endothelial growth factor and recombinant human bone morphogenetic protein 2 applied in spinal fusion

ABSTRACT

BACKGROUND:

Bone morphogenetic protein 2 possesses osteoinduction, and vascular endothelial growth factor plays a positive regulatory role in osteogenesis and bone repair. So what wil happen if both of them are used for spinal fusion?

To investigate the effects of fibrin glue combined with vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 on intertransverse process fusion.

Thirty Japanese white rabbits were randomized into three groups (n=10 per group) rabbits underwent intertransverse process fusion at the level of L5-6 with vascular endothelial growth factor 165/recombinant human bone morphogenetic protein 2/fibrin glue in experimental group, recombinant human bone morphogenetic protein 2/fibrin glue in control group, and fibrin glue in blank control group, respectively. At 6 weeks after implantation, observations of biomechanics, X-ray imaging and histology were performed. RESULTS AND

Osteogenic fusion between transverse processes could be found in experimental and control groups except for the blank control group, which was better in the experimental group. The strength of osteogenic fusion in the experimental group was significantly stronger than that in the control group (P < 0.01). Besides, abundant chondrocytes, osteocytes, mature bone tissues and col agens could be found in the osteogenic fusion specimen of the experimental group, and numerous chondrocytes, osteocytes and mature bone tissues observed in the osteogenic fusion specimen of the control group. In conclusion, fibrin glue combined with vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 can promote osteogenic fusion.