Determination of Vitamin B6 and Fluocinonide in Compound Vitamin B6 Gel by HPLC / 中国药师
China Pharmacist
;
(12): 1214-1216, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-494956
ABSTRACT
Objective:
To establish a method to determine vitamin B 6 and fluocinonide in compound vitamin B 6 gel.Methods:
The contents of vitamin B6 and fluocinonide in the gel were determined by HPLC with a Lichrospher CN C 18column (250 mm ×4.6 mm, 5μm).The mobile phase was heptane sulfonate solution (adding 6.8 g potassium phosphate monobasic and 1.0 g heptane sulfonate into 1000 ml water) -methanol –triethylamine (35650.2,adjusting pH to 3.2 with phosphoric acid).The detection wavelength was 238 nm and the column temperature was 25℃.The injection volume was 20 μl.Results:
Vitamin B6 had a good linear relationship within the range of 15.0-300.0 μg· ml-1(r=1.000 0), and the average recovery was 99.65% (RSD=0.3, n=9).Fluocinonide had a good linear relationship within the range of 0.4-8.0 μg· ml-1 (r=0.9990), and the average recovery was 99.35% (RSD=0.85, n=9).Conclusion:
The method is simple and reproducible, and the result is accurate.The method can be used for the deter-mination of the gel.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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