Study on polarization of macrophage in peripheral blood of patients with asthma / 中国免疫学杂志

ABSTRACT

Objective:To investigate the different function of macrophage between patients with asthma and healthy control , and discuss the pathogenesis of the disease .Methods:Choose 50 healthy controls ,50 patients with asthma , and isolation of blood mac-rophage.Then samples were induced with GM-CSF, and cells were collected on day 7.Macrophage function was evaluated using the following methods:fluorescence-activated cell sorting analysis for cell surface markers and phagocytosis ;enzyme-linked immunosorbent assay for cytokine production and the induction of T cell .Results: Compared with the control group , asthma group had decreased CD64, CD11c, CD40 expression, and increased CD206, CD14, CD23 expression(P<0.05);displayed the decreased IL-10 level(P<0.05 );displayed a decreased phagocytosis ability ( P<0.05 );when co-cultured with T cells , the supernatantdisplayed the increased IL-4 expression , and decreased IFN-γlevel ( P<0.05 ) .Conclusion: Compared with the control group , macrophage in asthma group displayed M2 phenotype, which inditates macrophage play the important role in the pathogenesis of asthma .