Simultaneous Determination of Three Flavonoids in Tianjihuang Granules by HPLC / 中国药师
China Pharmacist
;
(12): 1415-1417, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-495154
ABSTRACT
Objective:
To establish an HPLC method for the simultaneous determination of three flavonoids in Tianjihuang gran -ules.Methods:
A ZORBAX Eclipse XDB-C18 column was used.The mobile phase was methanol-0.2%phosphoric acid solution (42∶58).The detection wavelength was 350 nm and the flow rate was 1.0 ml· min-1.Results:
The linear range of isoquercitrin, quer-citrin and quercetin was 4.572-114.300 μg· ml-1(r=0.999 8), 7.028-175.700 μg· ml-1(r=0.999 8) and 2.195-54.880 μg· ml-1(r=0.999 9), respectively.The average recovery was 98.01%(RSD=1.24%, n=6), 98.14%(RSD=0.98%, n=6) and 97.00%(RSD=1.09%, n=6) , respectively.Conclusion:
The method is specific and simple,which can be used for the quality con-trol of Tianjihuang granules.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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