Digital holographic mi cor scopyf or detectin g galangin-induced apopt osis of HepG2 cells / 医学研究生学报

ABSTRACT

Objective Galangin has various antitumor activities and digital holographic microscopy ( DHM ) .This study aimed to investigate the detection of galangin-induced apoptosis of HepG2 cells using DHM. Methods HepG2 cells were cultured in vitro and treated with galangin at 130 μmol/L for 6, 12, and 24 hours respectively to induce apoptosis.Then, MTT assay was used to detect the proliferation of the HepG2 cells, Hoechst 33342 staining and flow cytometry adopted to determine their apoptosis, and DHM employed to visualize their morphological changes. Results Compared with the blank controls, galangin significantly inhibited the proliferation of the HepG2 cells ([7.32 ±2.47]%vs [39.14 ±2.04]%, P<0.01) and obviously induced their nuclear condensa-tion and apoptosis, with a total apoptosis rate of (11.550 ±0.043)%at 24 hours.DHM showed statistically significant differences be-tween the galangin-treated and control groups in the morphological changes of the HepG2 cells at the three time points (P<0.05). Conclusion Digital holographic microscopy can be used to detect the morphological changes of HepG2 cells during galangin-induced apop-tosis.