Platelet derived growth factor down-regulated sarcoplasmic reticulum Ca2+atpase to promote the phenotype modulation of human airway smooth muscle cells / 实用医学杂志
The Journal of Practical Medicine
;
(24): 2133-2137, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-495642
ABSTRACT
Objective To investigate of the effect and mechanisman of SERCA2 on the phenotype modulation of HASMCs. Methods HASMCs were starved for 5 days and divided into different groups ,then we observed morphology change of the cells from the microscope and detected a-actin、SERCA2 and p-ERK by Western Blot,cells proliferation was observed by CCK-8 method. Results Compared with the control group,PDGF could reduce a-actin of HASMCs and increased the cells proliferation ability ,TSG could significantly inhibit the effect (P<0.01), PDGF could also significantly inhibit SERCA2 protein and increased the expression p-ERK (P<0.01), while U0126 significantly inhibited the effect (P < 0.01). Conclusion PDGF may induce HASMCs phenotype modulation through the regulation of SERCA2 and p-ERK.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
The Journal of Practical Medicine
Year:
2016
Type:
Article
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