Protection of tea polyphenols against pulmonary ischemia-reperfusion injury in rats / 国际中医中药杂志

ABSTRACT

Objective To investigate the protection of tea polyphenols (TP) against pulmonary ischemia-reperfusion injury in rats. Methods A total of 100 rats were randomly devided into five groupsthe sham operation group, the model control group, the high-, medium-and low-dose TP groups. Two hours after reperfusion, the PO2 and the lung tissue wet/dry (W/D) ratio were examined; the apoptosis index (AI) was analysised;the activity of SOD, CAT and the content of MDA in lung tissue were determined. Results Compared with the model control group, the W/D (5.7 ± 0.4, 5.5 ± 0.4 vs. 6.5 ± 0.5) and the AI (19.3%± 1.8%, 14.2%± 1.4%vs. 31.2%± 2.4%) of the high-, medium-dose groups were significantly decreased (P<0.05 or P<0.01);the activity of SOD (115.2 ± 18.1 U/mg, 128.7 ± 20.9 U/mg vs. 94.7 ± 12.1 U/mg), CAT (1.3 ± 0.2 U/mg, 1.7 ± 0.4 U/mg vs. 0.9 ± 0.2 U/mg) in lung tissue of the the high-, medium-dose groups groups were significantly increased (P<0.05 or P<0.01), and the content of MDA (1.1 ± 0.3 nmol/L, 1.0 ± 0.3 nmol/L vs. 1.6 ± 0.5 nmol/L) were significantly decreased (P<0.05 or P<0.01). The PO2 (93.4%± 4.0%vs. 85.9%± 3.4%) of the high-dose group were significantly increased compared to the model group (P<0.01). Conclusion TPs could effectively lower the W/D, increase the PO2, inhibit the hepatocyte apoptosis, lower the AI, suggesting that TPs had protective effects against pulmonary ischemia-reperfusion injury in rats, which perhaps related to its effects of improving antioxidant ability and inhibiting the oxidative stress.