Effects of self-designed Chinese medicine decoction on the expression of c-myc and ;caspase-3 in the rats with precancerous lesion of chronic atrophic gastritis / 国际中医中药杂志

ABSTRACT

Objective To investigate the effects of self-designed Chinese medicine decoction on the expression of c-myc, caspase-3 in the rats with precancerous lesion of chronic atrophic gastritis. Methods The 120 Wistar rats were randomly divided into the normal group, the model group, the Weifuchun group and the high-, medium-and low-dose decoction groups, with 20 rats each group. Except the normal group, the rats in the other groups were prepared for chronic atrophic gastritis precancerous lesion model. After establishing the models successfully, the rats of the high-, medium-and low-dose groups were given decoction via intragastric administration which contained crude drug 2.088, 1.044, 0.522 g/ml respectively, and the Weifuchun group was given drug liquid of Weifuchun with the concentration of 0.076 g/ml. The normal group and the model group were given the equal volume of normal saline. Medicines were given once a day for 12 weeks. Then the expression of c-myc and caspase-3 in the gastric mucosa of rats were detected by using immunohistochemical staining. Results Compared to the model group, the expression of c-myc in the high-, medium-dose decoction groups and the Weifuchun group (30.25%± 3.56 %, 45.37% ± 4.81%, 38.79%± 4.02% vs. 63.45% ± 7.08%) significantly decreased (P<0.01 or P<0.05);and the expression of caspase-3 (36.85%± 5.02%, 31.24%± 4.55%, 31.57%± 4.62%vs. 23.43%± 2.95%) significantly increased (P<0.01 or P<0.05). Conclusions Self-designed Chinese medicine decoction could inhibit the expression of c-myc, and activate caspase-3 expression,which may be one of the mechanisms of treating the disease.