Pathogens distribution and drug resistance from cerebrospinal lfuid culture from 2007 to 2014 / 临床儿科杂志

ABSTRACT

Objective To explore the common pathogens distribution and the drug resistance pattern in vitro from cerebrospinal lfuid culture in children. Methods The results of cerebrospinal lfuid culture in hospitalized children from January 2007 to December 2014 were retrospectively analyzed. Bacteria identiifcation and antimicrobial susceptibility were assayed by Vitek system from Bio Mrieux Company. Some bacterial strains were tested by disk diffusion method. Results Cerebrospinal lfuid from 23099 patients were tested at least one time during research period. 671 strains of bacteria were isolated with positive rate of 2 . 9%, among which there were 579 strains of gram positive bacteria ( 86 . 3%) and 92 strains of gram negative bacteria ( 13 . 7%). The top ifve bacteria were coagulase negative Staphylococcus ( 399 strains, 58 . 9%), Micrococcus ( 37 strains, 5 . 5%), Streptococcus pneumoniae (34 strains, 5.1%), Escherichia coli (32 strains, 4.8%), and Enterococcus faecium (26 strains, 3.9%). The positive rates of cerebrospinal lfuid culture in 8 consecutive years showed a downward trend (χ2?=?10 . 410 , P=?0 . 001 ). The positive rates of coagulase negative Staphylococci showed annual decline trend (χ2?=?31 . 200 , PEscherichia coli showed an upward trend (χ2?=?4 . 786 , P=?0 . 029 ). The resistant rate of coagulase negative Staphylococcus to oxacillin was 79 . 8%, and no coagulase negative Staphylococcus resistant to linezolid and vancomycin was found. Micrococcus sensitive to vancomycin was 100%. The resistant rate of Streptococcus pneumoniae to penicillin was 77 . 8%, while the sensitive rate of Streptococcus pneumoniae to vancomycin was 100%. The sensitive rate of Escherichia coli to piperacillin/tazobactam and meropenemwas 100%. Conclusions The positive rate of cerebrospinal fluid culture is low. The main strains are coagulase negative Staphylococcus, Micrococcus, Streptococcus pneumoniae, Escherichia coli, and Enterococcus faecium. The antibiotics should been rationally chosen according to the results of bacterial strain culture and drug sensitive test.