Effects of iridoid on spermatogenesis of nicotinamide-streptozotocin induced type-2 diabetic mice / 天津医药

ABSTRACT

Objective To investigate the effects of iri doid on the spermatogenesis in mice of type-2 diabetes induced by nicotinamide and streptozotocin. Methods Fifty-six male C57BL mice were randomly divided into seven groups (n=8 for each group)control group, diabetic group, diabetic and glibenclamide (0.25 mg/kg) group, diabetic and iridoid (20 and 40 mg/kg) group, and iridoid (20 and 40 mg/kg) group. Diabetic model was induced by intraperitoneal injection of streptozotocin (65 mg/kg) and nicotinamide (120 mg/kg). Glibenclamide and iridoid were orally fed for 28 days. Twenty-four hours after the last treatment, blood samples, testis and the epididymis were collected for detecting blood hormones, testicular biopsy, and the evaluation of sperm parameters. Results The body weights of diabetic mice were recovered from the reduction after treatment with 40 mg/kg of iridoid and glibenclamide(P diabetic mice, and effectively improve the fertility of mice.