Primary culture of rabbit fibroblast-like synoviocytes using suspended explant culture method / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1009-1012, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-496533
ABSTRACT
Objective:
To isolate rabbit fibroblast-like synoviocytes(FLSs) by suspended explant culture method.Methods:
The healthy rabbit joint synovial layers were obtained. The cells were cultured by suspended explant culture method and compared with the explants adherent culture method. The growth status and morphology were observed. The growth curve of the 3rd passage cells was measured by CCK-8 assay,and the expression of vimentin was tested by immunocytochemistry.Results:
The FLS obtained by the two methods exhibited a spindle-shaped appearance and could rapidly expand. The cell growth curve was typical of S type, and the cells highly expressed vimentin.Conclusion:
Primary culture of rabbit fibroblast-like synoviocytes by suspended explant culture method were successfully established. The method was simple and highly efficient. It provided a new method for the isolation of FLS in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2016
Type:
Article
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