Effect of leptin on behavior and Bcl2 protein expression in rats with neonatal seizures / 中华行为医学与脑科学杂志

ABSTRACT

Objective To investigate the protective effects of leptin on neonatal seizure in rats by behavioral tests and Bcl2 expression.Methods Twenty Sprague-Dawley (SD) rats were divided into four groups:control group,leptin group,RS group and RS+leptin group on postnatal day 6 (P6),5 rats in each group.From P6 to P12,repeated neonatal seizure model was induced by flurothyl in RS and RS+leptin group rats.From P13 to P22,leptin (2 mg · kg-1 · day-1) was administered by intraperitoneal injection in leptin group and RS+leptin group rats.Forelimb hanging test and open field test were implemented on P30.Bcl2 expression was detected by western blot on P34.Results (1) Neurobehavioral tests:the time of forelimb suspension in RS group((7.10± 1.02) s)was significantly shorter (P＜0.05) than the control group((15.95± 6.11)s) and the time of forelimb suspension in RS+leptin group((13.90±2.64) s)was significant longer (P ＜0.05) than the RS group.In open field test,the number of horizontal movement in RS group (119.80± 28.83) was significantly reduced (P＜0.05) than that in the control group(191.00±37.27) and the number of horizontal movement in RS+leptin group(164.20±26.46) was increased than that in the RS group,but the difference was not significant.The number of modification and stool in RS group was significantly increased (P＜0.05) than that in the control group and the number of modification and stool in RS+leptin group was significantly reduced (P＜0.05) than that in the RS group.(2)Western blot:the expression of Bcl2 in RS group (0.24±0.07),leptin group(0.89±0.09) and RS+leptin group(0.56±0.07) was significantly decreased compared with control group(1.02±0.01) (P＜0.05),and the expression of Bcl2 in RS +leptin group was significantly increased compared with RS group (P＜0.05).Conclusion Leptin improves the brain injury induced by flurothyl on neonatal rats by Bcl2 signaling pathway.