Entrapment efficiency of cinobufagin and resibufogenin in toad skin extract loaded solid lipid nanoparticles:a comparison between centrifugation method and dialysis method / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 744-747, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-498168
ABSTRACT
Objective To establish an optimized method to determine the entrapment efficiencies(EE)of cinobufagin(CBG) and resibufogenin(RBG)in the toad skin extract loaded with solid lipid nanoparticles. Methods Dialysis,high-speed centrifugation and low-speed centrifugation were selected to determine the entrapment efficiencies of toad skin extract loaded solid lipid nanoparti?cles. The effects of different dialytic media on the entrapment efficiencies were investigated ,then the EE of every method were com?pared. Results Different methods had different results of EE,the EE of low-speed centrifugation,high-speed centrifugation and dialysis method in CBG and RBG were(74.00±1.69)%and(75.01±2.05)%,(83.60±0.99)%and(82.51±1.56)%,(91.01±0.75)%and(89.22± 0.88)%,respectively. The EE determined by different dialytic media of dialysis were different,but the results did not have the signifi?cant differences. Conclusion Different determination methods of EE have some significant influences on the results of EE,dialysis method is more suitable for the determination of EE for CBG and RBG in the toad skin extract loaded solid lipid nanoparticles.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2016
Type:
Article
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