Effect of cIAP1 down-regulation mediated by shRNA on biological behavior of human ovarian cancer cells / 实用肿瘤学杂志
Practical Oncology Journal
;
(6): 17-21, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-499257
ABSTRACT
[Abstratc] Objective The function of cIAP1 in the progression of ovarian cancer has not been clarified . This study is to explore the involvement of cIAP 1 in regulating biological behaviors of ovarian cancer cells by u-sing RNA interference(RNAi)technology.Mte hods The short hairpin RNA plasmid targeting cIAP1 was con-structed and transfected into Skov 3 cells.The levels of cIAP1 mRNA and protein were investigated by RT -PCR and Western Blot respectively .MTT assay and flow cytometry were used to evaluate cell proliferation and apopto-sis.R esults The rate of cIAP1 transfection was 74.7%performed by flow cytometric analysis .cIAP1 expression was significantly down -regulated at both mRNA and protein levels ,which resulted in a decrease of cell prolifera-tion and invasion capability in vitro .Conclusion This study implies that cIAP 1 might play an important role in the progression of ovarian cancer ,and it could be a potential target for therapeutic anti -cancer drugs .
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Practical Oncology Journal
Year:
2015
Type:
Article
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